Samson joined Le Tigre in 2000 as a replacement for Sadie Benning after working as the band's projectionist. She is also a member of the band The New England Roses and a member of electro-punk artist Peaches' live band, The Herms with Radio Sloan and Samantha Maloney. In 2006, Samson collaborated with Junior Senior on the song "Can I Get Get Get".
Samson's contributions to Le Tigre include the song "Viz," which deals with her experiences as a member of a sexual minority. In 2004, New York Times music writer Kelefa Sanneh called her "an icon of nerdy cool."
Samson is a co-founder of the performance art group "Dykes Can Dance." In 2003, she released 'JD's Lesbian Calendar', a collaboration with photographer Cass Bird, which was followed up in 2006 with 'JD's Lesbian Utopia,' a calendar documenting Samson's travels around the U.S. in an RV.
As of 2007, Samson is working with Le Tigre bandmate Johanna Fateman on a remix project titled MEN. JD co-produced with Bitch the Ferron song, "In the Meantime" from the CD Boulder released in 2008.
Samson also appeared in John Cameron Mitchell's Shortbus as "Jid", a patron of the Shortbus club.
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