Finding Dory will feature a transgender character, Ellen DeGeneres has confirmed.
DeGeneres, who voices the amnesiac blue tang fish in the film, told USA Today that “There’s a stingray that’s becoming sting-Rhonda, so there’s a trans sting in the movie.”
There has also been much speculation over whether the film will include Disney’s first same-sex couple after a new clip was released last month.
One scene in the latest trailer appears to show a girl with parents that are both women, sparking suggestion that the film could mark a progressive milestone in Pixar’s history.
However, DeGeneres claimed she was unaware whether the rumours were true.
“I don’t mind the question and I don’t know if its true or not, or if she has a bad short haircut,” DeGeneres said.
She added: “Who knows if she is a lesbian?”
Finding Dory director Andrew Stanton recently said of the pair: “They can be whatever you want them to be”.
The speculation comes amid calls for Disney to include gay and LGBT characters in their films.
Earlier this month, the Twitter hashtag #giveElsaAGirlfriend called on Disney to include a lesbian relationship in the upcoming sequel to Frozen.
The campaigns have also spread to live action films in the Disney canon, with the emergence of #GiveCaptainAmericaABoyfriend – which called for a gay relationship in upcoming Marvel films.