The performance will take place at Festival No. 6, which is held in Portmeirion between September 1-4.
Church and Rose will be joined on the festival’s main stage by Jacqui Abbott and Nadine Shah. The four women will perform some of the late star’s biggest hits, backed by the Manchester Camerata Orchestra.
Conductor and composer-in-residence Joe Duddell told BBC 6Music he had chosen to include only women in the piece because of Bowie’s “voice and his image”.
“He was a very androgynous figure and, certainly in the early music, his voice is very high,” he said. “If we were going to keep the songs in the same key, I was thinking that female singers would be the way forward – and we’ve stuck to that.
“It’ll be fascinating to see what their interpretations of the songs will be.”