The singer collaborated with Queen guitarist Brian May on a version of her recent single ‘Yoü And I’ at the ceremony, which was held at the Nokia Theatre in Los Angeles.
Gaga later delivered a monologue as the character as she accepted the Best Female Video award for her track ‘Born This Way’. The singer commented:
I’m so happy to accept the award tonight because Gaga’s not here for Best Female Video.
I feel so blessed to be here, and it’s true. It doesn’t matter who you are. Gay, straight, bi, lesbian, transgender – you were born this way. God bless you, MTV.
It’s not the first time Gaga has caused a stir at the VMAs – last year she turned up to the show wearing a meat dress.
Elsewhere at the ceremony, Beyonce confirmed she is pregnant with her first child, while there were also performances from the likes of Lil Wayne, Bruno Mars and ‘Watch The Throne’ collaborators Jay-Z and Kanye West.
See MTV.com for the full list of winners.