Playing an early evening slot on the Other Stage, the producer-turned-performer and his backing band the Version Players – including strings – played for the duration as his guests hopped on and off stage.
Ronson was then joined by “my friend Tiggers” who supplied vocals on Britney Spears’ ‘Toxic’ before Plastic Little burst onstage to supply an additional rap.
The duo stayed onstage to play ‘Ooh Wee’ from Ronson‘s first album, while the producer played cow bell.
Star Trak-signed vocalist Kenna was next up, performing his track ‘Out Of Control’, he then stayed on scaling the stage’s rigging to provide vocals on Radiohead‘s ‘Just’.
“Are you having a good time so far?” asked Ronson as Candie Payne joined in to sing her own ‘I Wish I Could Have Loved You More’.
“Does anyone like Coldplay? Does anyone like trumpets?” then asked Ronson before tearing into his instrumental of the band’s ‘God Put A Smile On Your Face’.
He then introduced The Rumble Strips‘ Charlie Waller to perform his band’s version of ‘Back To Black’ which the producer declared was “kinda better then the version Amy [Winehouse] and I wrote”.
They then performed The Jam‘s ‘Pretty Green’ together, until vocalist Taiwah took over the vocals halfway through the song.
She then sung the vocals for ‘Valerie’ as Ronson paid tribute to The Zutons, who will play the same stage later tonight.
Having introduced his band, Ronson told the crowd: “The whole point of Glastonbury is you’re supposed to do things you’ve never done before, I wrote this song with a friend of mine – is it OK if I bring her out to sing it?”
To loud screams the pink-haired Allen finally took the stage, admitting it had “been a while” as she performed ‘Littlest Things’ with the aid of a lyric sheet and Ronson‘s hints.
Allen then explained mid-song: “My Nanny Allen died last night, this one is for her.”
The singer then stayed onstage to cover Kaiser Chiefs‘ ‘Oh My God’.
“I’ve been asking her to do a song with us for two years now,” explained Ronson afterwards. “This is the first time she’s done it. It must be because she loves you lot, thank you Glastonbury.”
Daniel Merriweather then took to the stage to perform the set’s climax, The Smiths‘ ‘Stop Me If You Think You’ve Heard This One Before’, while Ronson played guitar from the front row of the audience.
Mark Ronson and his guests played:
‘Apply Some Pressure’
‘Out Of Control’
‘I Wish I Could Have Loved You More’
‘God Put A Smile On Your Face’
‘Back To Back’
‘The Littlest Things’
‘Oh My God’
‘Stop Me If You Think You’ve Heard This One Before’
Keep up with all the action from Glastonbury this weekend (June 27-29) as it happens on NME.COM. For news, pictures and blogs keep checking the NME.COM’s Glastonbury Festival page. Plus make sure you get next week’s issue of NME – on UK newsstands from July 2 – for the ultimate Glastonbury review.