Frontman Ian Broudie closed the band’s Pyramid Stage set with the track, which saw the large crowd rising to their feet to singalong.
“So there’s this little thing where England are taking on the whole world,” Broudie told the audience before the song. “Does anyone still believe?! I still believe! We can beat Germany!”
With that, he launched into an acoustic version of the track, a new version of which has been re-released for the current campaign.
He ended the song by saying: “Good luck England – beat Germany, beat Argentina, win the World Cup.”
England and Germany play at 3pm (BST) tomorrow.
The band’s set also featured tracks spanning their career, with the likes of ‘Change’, ‘Lucky You’, ‘The Life Of Riley’ and a cover of The Ronettes‘ ‘Be My Baby’ going down well.
Broudie also singled out 1989 single ‘Pure’ as being one of his favourite tracks from his back catalogue.
“This next song is the first song I ever wrote for The Lightning Seeds, and it’s still my favourite,” he declared.
The Lightning Seeds played:
‘Song For No One’
‘Be My Baby’
‘You Showed Me’
‘The Life Of Riley’
‘Sugar Coated Iceberg’